Recombinase Polymerase Amplification – The Breakthrough Isothermal Alternative to PCR
TwistDx’s proprietary technology, recombinase polymerase amplification (RPA), is transforming our ability to amplify and detect nucleic acids in laboratory and resource-limited field settings.
TwistDx’s isothermal nucleic acid amplification technology, Recombinase Polymerase Amplification (RPA), represents a hugely versatile alternative to polymerase chain reaction (PCR) for the development of fast, portable, nucleic acid detection assays. Inherently adaptable to applications as diverse as infectious disease diagnostics and food contamination tests, RPA is ideally suited to field, point-of-care and other settings with minimal resources, and particularly to situations where speed is essential. Easily transportable, user friendly and highly sensitive, RPA is as specific as PCR amplification but is much, much faster. Results are typically generated within 3-10 minutes. And unlike PCR, the RPA reaction doesn’t require thermal or chemical melting, so there’s no need for an expensive thermoycler or any additional equipment or reagents.
RPA is very forgiving of operating temperature. The reaction works optimally at a temperature of around 37-42˚C, which is lower than for other isothermal approaches, but will also work over a wide range of ambient temperatures. The dry-formulated RPA reagents exhibit excellent stability at ambient temperatures typically at least 12 months*. Refrigeration for short-term transport is unnecessary. The lyophilized reagent pellet also involves a simple workflow that can be carried out without specialist training.
RPA can be used to replace PCR in a wide variety of applications. End-users can easily design their own ultra-sensitive assays using their own primers. RPA technology can be adapted to a range of microfluidic, lateral flow and other devices, and by adding reverse transcriptase to the reaction mix RPA technology can also be used to amplify and detect RNA.